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Convulxin

Description:
Convulxin, a heterodimeric C-type lectin isolated from Crotalus durissus terrificus venom, activates mammalian platelets via binding and clustering of GPVI-receptors under physiological conditions. Occupation and clustering of GPVI activates Src family kinases, phosphorylating Fc receptor γ-chain and activating p72SYK that is critical for downstream activation of platelets. 

Application: Studies on platelet receptors. 

Origin: Crotalus durissus terrificus snake venom

MW: 84'000 

Assay:
The assay is performed in an aggregometer 
485 µl platelets suspension (5 x 108 platelets/ ml) 
=> are incubated for 1-2 min at 37°C, until base line is stable 
Add 5 µl CaCl2 200 mM 
Add 5 µl MgCl2 200 mM 
=> wait until base line is stable 
Add 5 µl collagen solution 0.5 mg/ml (positive control)
or
Add 5 µl Convulxin 0.1 µg/ml (diluted in HEPES buffer) 
=> measure aggregation at 37°C 

Inhibitors: Prostaglandins are strong inhibitors of convulxin-induced aggregation. 

Stability: Lyophilised in vials  -  2 years at -20°C 

Storage:
May be used until the expiry date given on the label when stored unopened, protected from moisture, in the dark, below -18 to -20°C. Avoid contamination of the reagents with micro organisms. Shipment of product does not require cooling during transportation.

Storage after reconstitution:
+15 to +25°C  -  8 hours 
+2 to +8°C  -  2 days 
-80°C  -  1 month 
The reconstituted product can be frozen immediately and stored at -80°C. Avoid refreezing.


Category: Research use only

Type: Snake Venom Protease Activator

Product Availability: Germany, Israel and Egypt

Manufacturer: DSM Nutritional Products Ltd. Branch Pentapharm, Switzerland

For more information please click .pdf icon below.


Convulxin

50 µg/vial
Artikelnr.: 811902

Einheit: 1 vial

Code: 119-02

Hersteller: DSM Nutritional Products Ltd. Branch Pentapharm

References:
 
  1. M. Cicmil, J.M. Thomas, T. Sage, F.A. Barry, M. Leduc, C. Bon, J.M. Gibbins. Collagen, convulxin, and thrombin stimulate aggregation-independent tyrosine phosphorylation of CD31 in platelets. Evidence for the involvement of Src family kinases. J Biol Chem 2000; 275:27339-47
  2. F. Niedergang, A. Alcover, C.G. Knight, R.W. Farndale, M.J. Barnes, I.M. Francischetti, C. Bon,
    M. Leduc. Convulxin binding to platelet receptor GPVI: competition with collagen related peptides. Biochem Biophys Res Commun 2000; 273:246-50
  3. I.M. Francischetti, B. Saliou, M. Leduc, C.R. Carlini, M. Hatmi, J. Randon, A. Faili, C. Bon. Convulxin, a potent platelet-aggregating protein from Crotalus durissus terrificus venom, specifically binds to platelets. Toxicon 1997; 35:1217-28
  4. J. Polgar, J.M. Clemetson, B.E. Kehrel, M. Wiedemann, E.M. Magnenat, T.N.C. Wells, K.J. Clemetson. Platelet activation and signal transduction by Convulxin, a C-type lectin from Crotalus durissus terrificus (tropical rattlesnake) venom via the p62/GPVI collagen receptor. The Journal of Biological Chemistry 1997; 272:13576-83

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