Convulxin, a heterodimeric C-type lectin isolated from Crotalus durissus terrificus venom, activates mammalian platelets via binding and clustering of GPVI-receptors under physiological conditions. Occupation and clustering of GPVI activates Src family kinases, phosphorylating Fc receptor γ-chain and activating p72SYK that is critical for downstream activation of platelets.
Application: Studies on platelet receptors.
Origin: Crotalus durissus terrificus snake venom
The assay is performed in an aggregometer
485 µl platelets suspension (5 x 108 platelets/ ml)
=> are incubated for 1-2 min at 37°C, until base line is stable
Add 5 µl CaCl2 200 mM
Add 5 µl MgCl2 200 mM
=> wait until base line is stable
Add 5 µl collagen solution 0.5 mg/ml (positive control)
Add 5 µl Convulxin 0.1 µg/ml (diluted in HEPES buffer)
=> measure aggregation at 37°C
Inhibitors: Prostaglandins are strong inhibitors of convulxin-induced aggregation.
Stability: Lyophilised in vials - 2 years at -20°C
May be used until the expiry date given on the label when stored unopened, protected from moisture, in the dark, below -18 to -20°C. Avoid contamination of the reagents with micro organisms. Shipment of product does not require cooling during transportation.
Storage after reconstitution:
+15 to +25°C - 8 hours
+2 to +8°C - 2 days
-80°C - 1 month
The reconstituted product can be frozen immediately and stored at -80°C. Avoid refreezing.
Category: Research use only
Type: Snake Venom Protease Activator
Product Availability: Germany, Israel and Egypt
Manufacturer: DSM Nutritional Products Ltd. Branch Pentapharm, Switzerland
For more information please click .pdf icon below.
Einheit: 1 vial
Hersteller: DSM Nutritional Products Ltd. Branch Pentapharm
- M. Cicmil, J.M. Thomas, T. Sage, F.A. Barry, M. Leduc, C. Bon, J.M. Gibbins. Collagen, convulxin, and thrombin stimulate aggregation-independent tyrosine phosphorylation of CD31 in platelets. Evidence for the involvement of Src family kinases. J Biol Chem 2000; 275:27339-47
- F. Niedergang, A. Alcover, C.G. Knight, R.W. Farndale, M.J. Barnes, I.M. Francischetti, C. Bon,
M. Leduc. Convulxin binding to platelet receptor GPVI: competition with collagen related peptides. Biochem Biophys Res Commun 2000; 273:246-50
- I.M. Francischetti, B. Saliou, M. Leduc, C.R. Carlini, M. Hatmi, J. Randon, A. Faili, C. Bon. Convulxin, a potent platelet-aggregating protein from Crotalus durissus terrificus venom, specifically binds to platelets. Toxicon 1997; 35:1217-28
- J. Polgar, J.M. Clemetson, B.E. Kehrel, M. Wiedemann, E.M. Magnenat, T.N.C. Wells, K.J. Clemetson. Platelet activation and signal transduction by Convulxin, a C-type lectin from Crotalus durissus terrificus (tropical rattlesnake) venom via the p62/GPVI collagen receptor. The Journal of Biological Chemistry 1997; 272:13576-83