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Pefabloc® SC, Inhibitor for Proteinase

Description:
Pefabloc® SC is an irreversible proteinase inhibitor with broad specificity for serine proteinases.

Application:
Pefabloc® SC is suitable for biopharmaceutical downstream purification because of its superior solubility, stability, inhibitory activity and low toxicity.

Formula: C8H10FNO2S · HCl

MW: 239.7

Chem. Name:
4-(2-Aminoethyl)-benzenesulfonylfluoride hydrochloride (AEBSF)

Solubility: 200 mg/ml in H2O in water

Storage:
May be used by the expiry date given on the label when stored unopened, protected
from moisture, in the dark, 2-8°C. Avoid contamination of the reagents by micro­
organisms.
Shipment of product does not require cooling during the time of transportation.

IC50 values:
The following table shows IC50 values for a selection of enzymes determined at pH 7.0, 25 °C after 15 minutes incubation time.

Enzyme Enyzme conc.     IC50 [mM]
Trypsin 0.2 µg/ml 0.081
Chymotrypsin 2.44 µg/ml 0.044
Thrombin 0.079 µg/ml 0.92
Factor Xa 0.12 U/ml 24.0
Plasmin 4 CTA-U/ml 1.99
tPA 5.0 µg/ml 0.72
uPA 24.0 µg/ml 0.072
Glandular kallikrein 3.5 µg/ml 2.86
Elastase 2.44 µg/ml 0.525
Subtilisin A 0.49 µg/ml 1.801
Factor XIIa 0.013 U/ml 0.256

Category: Research use only

Type: Inhibitor

Product Availability: Germany, Israel and Egypt

Manufacturer: DSM Nutritional Products Ltd. Branch Pentapharm, Switzerland

For more information please click .pdf icon below.


Pefabloc SC

Inhibitor for Proteinase
Artikelnr.: 839901

Einheit: 10 g

Code: 399-01

Hersteller: DSM Nutritional Products Ltd. Branch Pentapharm

Pefabloc SC

Inhibitor for Proteinase
Artikelnr.: 839901/g

Einheit: 1 g

Code: 399-01

Hersteller: DSM Nutritional Products Ltd. Branch Pentapharm

References:

  1. Chu TM, Kawinski E.
    Plasmin, subtilisin-like endoproteases, tissue plasminogen activator, and urokinase plasminogen activator are involved in activation of latent TGF-β1 in human seminal plasma.
    Biochem Biophys Res Commun 1998; 253: 128-34.
  2. Kaino S, Furui T, Hatano S, Kaino M, Okita K, Nakamura K.
    Twodimensional zymography for analysis of proteolytic enzymes in human pure pancreatic juice.
    Electrophoresis 1998; 19: 782-7.
  3. Mackarel AJ, Cottell DC, Russell KJ, Fitzgerald MX, O’Connor CM.
    Migration of neutrophils across human pulmonary endothelial cells is not blocked by matrix metalloproteinase or serine protease inhibitors.
    Am J Respir Cell Mol Biol 1999; 20: 1209-19.
  4. Mintz GR.
    An irreversible serine protease inhibitor.
    Bio Pharm 1993; 6: 34-8.
  5. Ofosu FA, Freedman J, Dewar L, Song Y, Fenton JW.
    A trypsin-like platelet protease propagates protease-activated receptor-1 cleavage and platelet activation.
    Biochem J 1998; 336: 283-5.
  6. Salamone PR, Wodzinski RJ.
    Production, purification and characterization of a 50-kDa extracellular metalloprotease from Serratia marcescens.
    Appl Microbiol Biotechnol 1997; 48: 317-24.
  7. Winton HL, Wan H, Cannell MB, Thompson PJ, Garrod DR, Stewart GA, Robinson C.
    Class specific inhibition of house dust mite proteinases which cleave cell adhesion, induce cell death and which increase the permeability of lung epithelium.
    Brit J Pharmacol 1998; 124: 1048-59.

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