Pefabloc® SC, Inhibitor for Proteinase
Description:
Pefabloc® SC is an irreversible proteinase inhibitor with broad specificity for serine proteinases.
Application:
Pefabloc® SC is suitable for biopharmaceutical downstream purification because of its superior solubility, stability, inhibitory activity and low toxicity.
Formula: C8H10FNO2S · HCl
MW: 239.7
Chem. Name:
4-(2-Aminoethyl)-benzenesulfonylfluoride hydrochloride (AEBSF)
Solubility: 200 mg/ml in H2O in water
Storage:
May be used by the expiry date given on the label when stored unopened, protected
from moisture, in the dark, 2-8°C. Avoid contamination of the reagents by micro
organisms.
Shipment of product does not require cooling during the time of transportation.
IC50 values:
The following table shows IC50 values for a selection of enzymes determined at pH 7.0, 25 °C after 15 minutes incubation time.
| Enzyme |
Enyzme conc. |
IC50 [mM] |
| Trypsin |
0.2 µg/ml |
0.081 |
| Chymotrypsin |
2.44 µg/ml |
0.044 |
| Thrombin |
0.079 µg/ml |
0.92 |
| Factor Xa |
0.12 U/ml |
24.0 |
| Plasmin |
4 CTA-U/ml |
1.99 |
| tPA |
5.0 µg/ml |
0.72 |
| uPA |
24.0 µg/ml |
0.072 |
| Glandular kallikrein |
3.5 µg/ml |
2.86 |
| Elastase |
2.44 µg/ml |
0.525 |
| Subtilisin A |
0.49 µg/ml |
1.801 |
| Factor XIIa |
0.013 U/ml |
0.256 |
Category: Research use only
Type: Inhibitor
Product Availability: Germany, Israel and Egypt
Manufacturer: DSM Nutritional Products Ltd. Branch Pentapharm, Switzerland
For more information please click .pdf icon below.
Pefabloc SC
Inhibitor for Proteinase
Artikelnr.: 839901
Einheit: 10 g
Code: 399-01
Hersteller: Pentapharm AG
Pefabloc SC
Inhibitor for Proteinase
Artikelnr.: 839901/g
Einheit: 1 g
Code: 399-01
Hersteller: Pentapharm AG
References:
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Plasmin, subtilisin-like endoproteases, tissue plasminogen activator, and urokinase plasminogen activator are involved in activation of latent TGF-β1 in human seminal plasma.
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Electrophoresis 1998; 19: 782-7.
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Migration of neutrophils across human pulmonary endothelial cells is not blocked by matrix metalloproteinase or serine protease inhibitors.
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- Mintz GR.
An irreversible serine protease inhibitor.
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- Ofosu FA, Freedman J, Dewar L, Song Y, Fenton JW.
A trypsin-like platelet protease propagates protease-activated receptor-1 cleavage and platelet activation.
Biochem J 1998; 336: 283-5.
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Production, purification and characterization of a 50-kDa extracellular metalloprotease from Serratia marcescens.
Appl Microbiol Biotechnol 1997; 48: 317-24.
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Class specific inhibition of house dust mite proteinases which cleave cell adhesion, induce cell death and which increase the permeability of lung epithelium.
Brit J Pharmacol 1998; 124: 1048-59.