anti-human CD43, Clone HI161

Description
CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin¬binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumour suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions.

Properties
The monoclonal antibody ADG5045/L (clone HI161) is a murine monoclonal antibody, subclass IgG₁. The antibody has been purified by protein-G affinity chromatography, Purity > 95% (by SDS-PAGE).

The antibody recognizes neuraminidase-sensitive epitope on CD43 (Leukosialin), a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes; it is negative on resting B lymphocytes and erythrocytes.

Presentation
Vial containing 100 µg /100 µl (ADG5045) or 300 µg/300 µl (ADG5045L) of purified antibody PBS containing 0.09 % sodium azide (pH 7.2) pH 7.2. The IgG concentration is 1 mg/ml. Spin the vial briefly before opening.

Storage and Stability
Store at 4 °C. For long-term storage aliquot and store at -20°. It is recommended to avoid freeze-thaw cycles. The reagent is stable until the expiry date stated on the vial label.

Applications
Flow Cytometry
Immunohistochemistry (acetone-fixed frozen sections and formalin-fixed paraffin sections)

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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