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anti-human CD147, FITC-conjugated, Clone MEM-M6/1

Description
CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6) also known as EMMPRIN (extracellular matrix metalloproteinase inducer) or TCSF (tumour cell-derived collagenase-stimulatory factor) is an ubiquitously expressed cell surface protein with multiple glycosylated forms. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumour cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.

Properties
The monoclonal antibody ADG5087/L (clone MEM-M6/1) is a murine monoclonal antibody, subclass IgG1. The antibody has been purified from ascites using protein-A affinity chromatography, Purity > 95% (by SDS-PAGE).

The antibody recognizes an epitope in the N-terminal Ig domain (D1) of CD147 (Neur¬othelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by resting lymphocytes. The antibody MEM-M6/1 is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.

Conjugation
The purified antibody is conjugated with Fluorescein-isothiocyanate (FITC) under optimum conditions. The reagent is adjusted for direct use. No reconstitution is necessary.

Presentation
Vial containing 500 µl (ADG5087) or 2 ml (ADG5087L) of purified antibody in PBS containing 1% BSA and 0.09% sodium azide (pH 7.2). The IgG concentration is 1 mg/ml. Spin the vial briefly before opening.

Storage and Stability
Store the antibody at 4°C. Avoid prolonged exposure to light. The reagent is stable until the expiry date stated on the vial label.

Applications
Flow Cytometry

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

For more information please click .pdf icon below.


anti-human CD147, FITC-conjugated, Clone MEM-M6/1

Cat.No. ADG5087
Artikelnr.: 938224

Einheit: 500 ml

Code: ADG5087

Hersteller: ImmBioMed GmbH & Co. KG

References

  1. Kirk P et al., EMBO J. 2000 Aug 1;19(15):3896-904.
  2. Wilson MC et al., J Biol Chem. 2005 Jul 22;280(29):27213-21.
  3. Xu D, Hemler ME: Mol Cell Proteomics. 2005 Aug;4(8):1061-71.
  4. Iacono KT et al., Exp Mol Pathol. 2007 Dec;83(3):283-95.
  5. Ruiz S et al., J Biol Chem. 2008 Feb 29;283(9):5554-66.
  6. Melchior A et al., Exp Cell Res. 2008 Feb 1;314(3):616-28.
  7. Schmidt R et al., Circ Res. 2008 Feb 15;102(3):302-9.

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