murine MAb against p83/100 (BB0744) of Borrelia burgdorferi, Clone LA-114.1
Description
Lyme disease is the most common vector-borne disease in North America and Europe. The causative agent Borrelia burgdorferi is a bacterium that is maintained in an enzoonotic cycle between Ixodes ticks and a large range of mammals. In Lyme disease, p83/100 is a known serodiagnostic antigen for Lyme infection. It is speculated that p83/100 is needed indirectly for the stabilization of an important cell envelope structure that provides adhesion to host cells.
Properties
The monoclonal antibody ADG0111L (clone LA-114.1) is a murine monoclonal antibody, subclass IgG1 recognizing p83/100. Mice were immunized with cell lysates of Borrelia burgdorferi. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.
Presentation
Screw capped vial containing 1 mg of purified antibody in PBS pH 7.4. The IgG concentration is given on the vial label. Spin the vial briefly before opening.
Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.
Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Westernblot
The antibody is suitable for Western blot analysis, detecting native and recombinant p83/100 following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Immunocytochemistry
The antibody can be used for immuno-cytochemistry on permeabilized and fixed spirochetes.
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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murine MAb against p83/100 (BB0744) of Borrelia burgdorferi, Clone LA-114.1
Cat.No. ADG0111L (research box)
Artikelnr.: 938297
Einheit: 1,0 mg
Code: ADG0111L
Hersteller: ImmBioMed GmbH & Co. KG - from the research box
References
- Molecular and immunological characterization of the p83/100 protein of various Borrelia burgdorferi sensu lato strains. Rössler et al. Med. Microbiol. Immunol 1995; 184(1):23-32
- Interpretation criteria for standardized Western blots for three European species of Borrelia burgdorferi sensu lato. Hauser et al. J. Clin. Microbiol. 1997;35(6):1433-1444
- Genospecies and their influence on immunoblot results. Wilske et al. Wien. Klin. Wochenschr. 1998; 110(24):882-885
- An improved recombinant IgG immunoblot for serodiagnosis of Lyme borreliosis. Wilske et al. Med. Microbiol. Immunol. 1999;188(3):139.144
- Microbiological and serological diagnosis of Lyme borreliosis. Wilske et al. FEMS Immunol. Med. Microbiol. 2007;49(1):13-21
- BB0744 affects tissue tropism and spatial distribution of Borrelia burgdorferi. Wager et al. Infect. Immun. 2015; 83(9):3693-3703