murine MAb against outer surface lipoprotein BBA03 of Borrelia burgdorferi, Clone LA-95.1
Description
Lyme disease is the most common vector-borne disease in North America and Europe. The causative agent Borrelia burgdorferi is a bacterium that is maintained in an enzoonotic cycle between Ixodes ticks and a large range of mammals. Linear plasmid lp54 is one of the most highly conserved and differentially expressed elements of the segmented genome of Borrelia burgdorferi. Several outer surface proteins encoded on the lp54 plasmid have been shown to be critical at specific times during the infectious cycle. Among these are the OspA/B, DbpA/B, BBA03, BBA52, and BBA64.
Properties
The monoclonal antibody ADG0118L (clone LA-95.1) is a murine monoclonal antibody, subclass IgG1 recognizing an outer surface 20 kDa lipoprotein (BBA03). Mice were immunized with cell lysates of Borrelia burgdorferi. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.
Presentation
Screw capped vial containing 1 mg of purified antibody in PBS pH 7.4. The IgG concentration is given on the vial label. Spin the vial briefly before opening.
Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.
Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Westernblot
The antibody is suitable for Western blot analysis, detecting native and recombinant lpLA7 following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Immunocytochemistry
The antibody can be used for immune-cytochemistry on paraformaldehyde fixed.
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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murine MAb against outer surface lipoprotein BBA03 of Borrelia burgdorferi, Clone LA-95.1
Cat.No. ADG0118L (research box)
Artikelnr.: 938308
Einheit: 1,0 mg
Code: ADG0118L
Hersteller: ImmBioMed GmbH & Co. KG - from the research box
References
- Competitive advantage of Borrelia burgdorferi with outer surface protein BBA03 during tick-mediated infection of the mammalian host. Bestor et al. Infect. Immun. 2012; 80(10):3501-3511
- Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi. Fraser et al. Nature 1997; 390:580-586
- Comprehensive spatial analysis of the Borrelia burgdorferi lipoproteome reveals a compart-mentalization bias toward bacterial surface. Dowdell et al. J. Bacteriol. 2017; 199(6):e00658-16
- Identification of 11 pH-regulated genes in Borrelia burgdorferi localizing to linear plasmids. Carroll et al. Infect. Immun. 2000; 68(12):6677-6684