murine MAb against outer surface lipoprotein BBA66 of Borrelia burgdorferi, Clone A66-EE
Description
Lyme disease is the most common vector-borne disease in North America and Europe. The causative agent Borrelia burgdorferi is a bacterium that is maintained in an enzoonotic cycle between Ixodes ticks and a large range of mammals. Several Borrelia burgdorferi proteins are upregulated by temperature- and/or mammalian host-specific signals as the spirochete is transmitted from ticks to mammals. BBA66 outer surface lipoprotein was found to be up-regulated during transmission and is immunogenic in mammals.
Properties
The monoclonal antibody ADG0120L (clone A66-EE) is a murine monoclonal antibody, subclass IgG1 recognizing BBA66. Mice were immunized with rec. BBA66 of Borrelia burgdorferi. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.
Presentation
Screw capped vial containing 1 mg of purified antibody in PBS pH 7.4. The IgG concentration is given on the vial label. Spin the vial briefly before opening.
Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.
Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Westernblot
The antibody is suitable for Western blot analysis, detecting native and recombinant BBA66 following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Immunocytochemistry
The antibody can be used for immunocytochemistry on paraformaldehyde fixed spirochetes.
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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murine MAb against outer surface lipoprotein BBA66 of Borrelia burgdorferi, Clone A66-EE
Cat.No. ADG0120L (research box)
Artikelnr.: 938311
Einheit: 1,0 mg
Code: ADG0120L
Hersteller: ImmBioMed GmbH & Co. KG - from the research box
References
- Identification of 11 pH-regulated genes in Borrelia burgdorferi localizing to linear plasmids. Carroll et al. Infect. Immun. 2000; 68(12):6677-6684
- Identification and functional characterization of complement regulator-acquiring surface protein 1 of the Lyme disease spirochetes Borrelia afzelii and Borrelia garinii. Wallich et al. Infect. Immun. 2005; 73(4):2351-2359
- Temporal expression analysis of the Borrelia burgdorferi paralogous gene family genes BBA64, BBA65, and BBA66 during persistent infection in mice. Gilmore et al. Infect. Immun. 2007; 75(6):2753-2764
- Fast, adaptive evolution at a bacterial host-resistance locus: the PFam54 gene array in Borrelia burgdorferi. Wywial et al. Gene. 2009; 445(1-2):26-37
- BBA70 of Borrelia burgdorferi is a novel plasminogen-binding protein. Koenigs et al. J. Biol. Chem. 2013: 288(35):25229-25243
- Crystal structure of the infectious phenotype-associated outer surface protein BBA66 from the Lyme disease agent Borrelia burgdorferi. Brangulis et al. Ticks Tick Borne Dis. 2014; 5(1):63-68
- Structural analysis of the outer surface proteins from Borrelia burgdorferi paralogous gene family 54 that are thought to be the key players in the pathogenesis of Lyme disease. Brangulis et al. J. Struct. Biol. 2020; 210(2):107490