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murine MAb against BhCRASP-1 of Borrelia hermsii, Clone Bh-3.1

Tick-borne relapsing fever in North America is primarily caused by the spirochete Borrelia hermsii. Spirochetes are transmitted to humans through the bite of infected soft ticks, in particular Ornithodoros hermsii. The pathogen employs multiple strategies, including the acquisition of complement regulators and antigenic variation, to escape innate and adaptive immune responses and to persist in the blood. The outer surface protein BhCRASP-1 binds the complement regulators factor H and FH-related protein 1. Furthermore, BhCRASP-1 binds plasminogen/plasmin and contributes to dissemination/invasion of B. hermsii.

The monoclonal antibody ADG0172L (Bh-3.1) is a murine monoclonal antibody, subclass IgG1 recognizing BhCRASP-1 of Borrelia hermsii strain HS1. Mice were immunized with rec. BhCRASP-1. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.

Vial containing 1 mg purified antibody in PBS pH 7.4. The concentration is given on the vial label. Spin the vial briefly before opening.

Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.

The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
The antibody can be used for immune-cytochemistry on paraformaldehyde fixed spirochetes
The antibody is suitable for Western blot analysis, detecting native and recombinant BhCRASP-1 following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Surface exposure of BhCRASP-1. (A) Borrelia hermsii after incubation with a BhCRASP-1-specific mAb Bh-3.1 followed by rabbit anti-mouse Cy3-conjugated IgG (left) and differential interference contrast image (right). (B) Proteinase K and trypsin treatment affects surface expression of native BhCRASP-1. B. hermsii cells were incubated with the indicated concentrations of proteinase K and trypsin, lysed by sonication, immunoblotted, and screened with anti-BhCRASP-1 mAb Bh-3.1

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

For more information please click .pdf icon below.

murine MAb against BhCRASP-1 of Borrelia hermsii, Clone Bh-3.1

Cat.No. ADG0172L (research box)
Artikelnr.: 938330

Einheit: 1,0 mg

Code: ADG0172L

Hersteller: ImmBioMed GmbH & Co. KG - from the research box


  1. Dual binding specificity of a Borrelia hermsii-associated complement regulator-acquiring surface protein for factor H and plasminogen discloses a putative virulence factor of relapsing fever spirochetes. Rossmann et al. J. Immunol. 2007; 178 (11):7292-7301
  2. BhCRASP-1 of the replasing fever spirochete Borrelia hermsii is a factor H- and plasminogen-binding protein. Rossmann et al. Int. J. Med. Microbiol. 2008; 298(S1):272-283

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