rat MAb against murine Granzyme A, clone HD-TSP 6.3
Description
The monoclonal antibodies ADG0466L (clone HD-TSP 6.2) and ADG0467L (clone HD-TSP 7.1) are directed against murine Granzyme A (murine T cell-specific serine protease 1, mTSP-1). Rats were immunized with purified Granzyme A isolated from long term culture cytolytic killer TLC cells (1.3E6SN). The antibodies have been purified from cell culture supernatant using Protein G affinity chromatography. The antibodies react with both native and recombinant Granzyme A.
Presentation
Screw capped vial containing 1 mg of purified antibody in PBS pH 7.4. The IgG concentration is given on the vial label. Spin the vial briefly before opening.
Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.
Applications
Western Blot
The antibodies recognize native (mTSP-1) and recombinant Granzyme A/fusion protein (FP) following SDS-PAGE under non-reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Immunohistochemistry
The antibody can be used for immune-histological staining of acetone fixed cryosections. A primary antibody concentration of 1-10 µg/mL is recommended.
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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rat MAb against murine Granzyme A, clone HD-TSP 6.3
Cat.No. ADG0466L (research box)
Artikelnr.: 938347
Einheit: 1,0 mg
Code: ADG0466L
Hersteller: ImmBioMed GmbH & Co. KG - from the research box
References
- Expression of cytoplasmic granules with T cell-associated serine proteinase-1 activity in Ly-2+(CDS+) T lymphocytes responding to lymphocytic choriomeningitis virus in vivo*. Kramer MD et al. Eur J Immunol. 1989 Jan;19(1):151-156.
- Expression of T-cell-associated serine proteinase 1 during murine Leishmania major infection correlates with susceptibility to disease. Moll H, Müller C, Gillitzer R, Fuchs H, Röllinghoff M, Simon MM, Kramer MD. Infect Immun. 1991 Dec;59(12):4701-4705.