murine MAb against human tPA
Description
The monoclonal antibody ADG3504 (clone HD-tPA 4.1) is a murine monoclonal antibody, subclass IgG1 recognizing the human Tissue-type Plasminogen Activator (tPA). Mice were immunized with purified native human tPA. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography. The antibody reacts with both human single-chain tPA, two-chain tPA.
Presentation
Screw capped vial containing 250 µg of purified antibody in PBS pH 7.4, 0.01 % ProClin300. The IgG concentration is given on the vial label. Spin the vial briefly before opening.
Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.
Applications
ELISA
The antibody is suitable as detection antibody in ELISAs. (1)
Inhibition
The antibody blocks tPA-mediated plasminogen activation in a chromogenic substrate assay. (1)
The antibody blocks the invasion of a human tPA-expressing melanoma cell line (MeWo) in an in vitro assay system. (2)
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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murine MAb against human tPA, clone HD-tPA 4.1
Cat.No. ADG3504
Artikelnr.: 938523
Einheit: 250 µg
Code: ADG3504
Hersteller: ImmBioMed GmbH & Co. KG
References
- Monoclonal antibodies against plasminogen activators and plasmin(ogen). MD Kramer, U Vettel, M Schmitt, J Reinartz, G Brunner, and A Meissauer. Fibrinolysis 1992;6, Suppl 4:103-111.
- Generation of cell surface-bound plasmin by cell-associated urokinase-type or secreted tissue-type plasminogen activator: a key event in melanoma cell invasiveness in vitro. Meissauer A, Kramer MD, Schirrmacher V, Brunner G. Exp Cell Res. 1992 Apr;199(2):179-901.