murine MAb against OspB Borrelia burgdorferi, Clone LA-25.1
Description
Borrelia burgdorferi outer surface protein OspB is expressed by spirochetes in the Ixodes scapularis gut. ospB is transcribed from a bicistronic operon with ospA, a known spirochete adhesion gene in the tick gut (1). Several reports, however, have indicated that OspB is present on the surface of B. burgdorferi within unfed ticks (2). Certain OspB antibodies, either as whole immunoglobulin G (IgG) or as Fab fragments, can be bactericidal in vitro, and studies have demonstrated that vaccination with OspB can protect mice from B. burgdorferi infection (3,4,5).
Preparation
The monoclonal antibody ADG84 (clone LA-25.1) is a murine monoclonal antibody, subclass IgG2b recognizing OspB. Mice were immunized with recombinant OspB antigen. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.
Presentation
Screw capped vial containing 250 µg of purified antibody in PBS pH 7.4 + 0.01% ProClin300. Spin the vial briefly before opening. The IgG concentration is indicated on the vial label.
Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.
Applications
• ELISA
• Westernblot
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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murine MAb against OspB Borrelia burgdorferi, Clone LA-25.1
Cat.No. ADG0084 (research box)
Artikelnr.: 938543
Einheit: 250 µg
Code: ADG0084
Hersteller: ImmBioMed GmbH & Co. KG - from the research box
References
- Organization of genes encoding two outer membrane proteins of the Lyme disease agent Borrelia burgdorferi within a single transcriptional unit. Howe TR et al., Infect Immun 1986, 54:207-212.
- Heterogeneity of Lyme disease spirochaetes within individual vector ticks. Brunet, LR et al., Res Microbiol 1997, 148:437-445.
- Characterization of the physiological requirements for the bactericidal effects of a monoclonal antibody to OspB of Borrelia burgdorferi by confocal microscopy. Escudero R et al., Infect. Immun 1997, 65:1908-1915.
- Evasion of protective immunity by Borrelia burgdorferi by truncation of OspB. Fikrig, E et al., Proc Natl Acad Sci USA 1993, 90:4092-4097.
- A bactericidal monoclonal antibody elicits a change in its antigen, OspB of Borrelia burgdorferi, that can be detected by limited proteolysis. Katona L et al., J Immunol 2000, 164:1425-1431.