murine MAb against OspC Borrelia burgdorferi, Clone LA-C1.3
Description
Osp C (Outer surface Protein C) is a major virulence factor of Borrelia burgdorferi, the causative agent of Lyme disease. OspC is detectable on spirochetes in ticks after engorgement, but also on spirochetes present in several mammalian tissues. OspC binds to an immunosuppressive tick saliva protein, termed Salp15, which allows the tick to evade and/or suppress host responses during engorgement for several days. The presence of OspC on B. burgdorferi in the mammalian host make it an attractive vaccine target antigen.
Properties
The monoclonal antibody ADG90L (clone LA-C1.3) is a murine monoclonal antibody, subclass IgG2b recognizing OspC. Mice were immunized with recombinant OspC antigen. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.
Presentation
Vial containing 1 mg purified antibody in PBS pH 7.4. The concentration is given on the vial label. Spin the vial briefly before opening.
Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.
Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Westernblot
The antibody is suitable for Western blot analysis, detecting native and recombinant OspC following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Immunocytochemistry
The antibody can be used for immunocytochemistry on paraformaldehyde fixed spirochetes and for cryo-electron tomography.
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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murine MAb against OspC Borrelia burgdorferi, Clone LA-C1.3
Cat.No. ADG0090L (research box)
Artikelnr.: 938553
Einheit: 1,0 mg
Code: ADG0090L
Hersteller: ImmBioMed GmbH & Co. KG - from the research box
References
- Induction of an outer surface protein on Borrelia burgdorferi during tick feeding. Schwan et al. Proc. Natl. Acad. Sci. USA 1995; 92:2909-2913
- Hepatitis B virus capsid-like particles can display the complete, dimeric outer surface protein C and stimulate production of protective antibody responses against Borrelia burgdorferi infection. Skamel et al. J. Biol. Chem. 2006; 281:17474-17481
- Resolution of experimental and tick-borne Borrelia burgdorferi infection in mice by passive, but not active immunization using recombinant OspC. Zhong et al. Eur. J. Immunol. 1999; 29:946-957
- The Lyme disease agent exploits a tick protein to infect the mammalian host. Ramamoorthi et al. Nature 2005; 436:573-577
- Crystal structure of outer surface protein C (OspC) from the Lyme disease spirochete, Borrelia burgdorferi. Kumaran et al. EMBO J. 2001; 20:971-978
- Immunological and molecularpolymorphisms of OspC, an immunodominant major outer surface protein of Borrelia burgdorferi. Wilske et al. Infect. Immun. 1993; 61:2182-2191
- Nanoscopic Localization of Surface-Exposed Antigens of Borrelia burgdorferi. Lemgruber et al. Microsc. Microanal. 2015; 21(03):680-688
- Comparative cryo‐electron tomography of pathogenic Lyme disease spirochetes. Kudryashev et al. Mol. Microbiol. 2009; 71(6):1415-1434