human Factor XIII-A2, recombinant
Description
Human Factor XIII was recombinantly produced in insect cells. Factor XIII is a homodimer (a2) composed of two chains held together by non-covalent bonds. After activation of the zymogen by Thrombin and Ca2+ to its active form (a*2 , Factor XIIIa), Factor XIIIa catalyzes the formation of covalent bridges (ε-(γ-glutamyl) lysine bonds) between fibrin units to increase the elasticity of the clot network. The resulting cross-linked fibrin is insoluble and resistant to lysis.
Synonym
Recombinant Fibrin stabilizing factor, protein-glutamine-γ-glutamyltransferase
Purity > 95% [by SDS-PAGE]
Molecular Weight 84 kDa (monomer)
168 kDa (homodimer)
Application
The FXIIIa catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue.
Presentation
200 µg of the recombinant human Factor XIII is lyophilized from 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM DTT. Sample contains maltodextrin.
Reconstitution
Add the volume of water specified in the certificate of analysis under aliquotation to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. For short term storage keep cooled on ice.
Storage
Store at –20 °C in working aliquots. Repeated freezing and thawing is not recommended.
Delivery at ambient temperature is possible
Category: Research use only
Type: Protein
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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human Factor XIII-A2, recombinant
Cat.No. ADG424
Artikelnr.: 938754
Einheit: 200 µg
Code: ADG424
Hersteller: ImmBioMed GmbH & Co. KG
References
- Novel insights into structure and function of factor XIIIa-inhibitor tridegin. Böhm M et al. J Med Chem. 2014 Dec 26;57(24):10355-65.
- Coagulation factor XIIIa substrates in human plasma: identification and incorporation into the clot. Nikolajsen CL et al. J Biol Chem. 2014 Mar 7;289(10):6526-34.
- Differences in the inhibition of coagulation factor XIII-A from animal species revealed by Michael Acceptor- and thioimidazol based blockers. Heil A et al. Thromb Res. 2013 May;131(5):e214-22.
- A profiling platform for the characterization of transglutaminase 2 (TG2) inhibitors. Schaertl S et al. J Biomol Screen. 2010 Jun;15(5):478-87.