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Pefachrome® PCa, Chromogenic Substrate for activated Protein C

Application:
Highly sensitive chromogenic peptide substrate for activated protein C. Determination of Protein Ca activity for research, in-process and quality control.

Formula: H-D-Lys(Cbo)-Pro-Arg-pNA · 2AcOH

Principle:
Protein C + Protac® ==> Protein Ca
H-D-Lys(Cbo)-Pro-Arg-pNA + PCa ==> H-D-Lys(Cbo)-Pro-Arg-OH + pNA + PCa

Solubility: Up to 4 mM in H2O

MW: 773.9

KM: 0.303 mM

vmax: 25.0 µmol/ml protein C/min

Storage:
May be used by the expiry date given on the label when stored unopened, protected from moisture, in the dark, 2-8°C. Avoid contamination of the reagents by micro-organisms. Shipment of product does not require cooling during the time of transportation.

Material required but not provided:
Reference material, buffer, Protac®

Buffer:
50 mM Tris-imidazole buffer pH 8.4, 150 mM NaCl

Assay:
Suggested protocol for the determination of Protac®-activated protein C:
0.050 ml citrated plasma
0.100 ml Protac®,0.5 units/ml
=> incubate for 5 min at 37°C
1.650 ml buffer 
0.200 ml Pefachrome®PCa, 4 mM in H2O
==>Determination of ∆OD/min at 405 nm

Category: Research use only

Type: Substrate

Product Availability: Germany, Israel and Egypt

Manufacturer: DSM Nutritional Products Ltd. Branch Pentapharm, Switzerland


For more information please click .pdf icon below.


Pefachrome PCa

Chromogenic Substrate for activated Protein C
Article no.: 808902/25

Unit: 25 mg

Code: 089-02

Manufacturer: Pentapharm AG

References

  1. Stocker K, Fischer H, Meier J.
    Practical application of the protein C activator Protac® from Agkistrodon contortrix venom.
    Folia Haematol 1988; 115: 260-64.
  2. Takahashi H, Hanano H, Tatewaki W, Shibata A.
    Fast functional assay of protein C in whole plasma using a snake venom activator: Evaluation in patients with congenital and acquired protein C deficiencies.
    Clin Chim Acta 1988; 175: 217-22.
  3. Wikstroem P, Svendsen L, Schulze AJ, Prasa D, Stuerzebecher J.
    Highly selective chromogenic and fluorogenic peptide substrates for activated protein C. Poster GTH 1998, Frankfurt, Germany

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