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murine MAb against human uPAR

Description
The monoclonal antibody ADG3937 (clone HD-UPAR-13.1.1) is a murine monoclonal antibody, subclass IgG1 recognizing the human urokinase receptor (uPAR) domain 2+3. It binds with high affinity to both uPAR and uPA/uPAR complexes.

Properties
ADG3937 is non-inhibitory, it does not block uPA from binding to uPAR. Pre-incubation with various uPAR preparations (CHO cell line, U937 cells) can completely inhibit binding of ADG3937 to uPAR). Cross-reactivity with uPAR from other species has not been determined.

Presentation
Screw capped vial containing 250 µg of purified antibody in PBS pH 7.4, 0.01 % ProClin300. The IgG concentration is 1 mg/ml. Spin the vial briefly before opening.

Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.

Applications
A. Immunohistochemical/Flow Cytometry Analysis
ADG3937 is suitable for staining of formalin-fixed, paraffin-embedded tissue sections. The antibody stains both fresh frozen and formalin-fixed PMA stimulated U937 cells for flow cytometry. A 1:100 dilution (10 μg/mL concentration) is recommended.
B. Immunoprecipitation of uPAR
Using a 5:1 molar ratio, ADG3937 precipitates various uPAR preparations, showing a broad band between 39-66 kDa per an SDS-gel electro-phoresis, owing to the extensive glycosylations of the preparations (N-linked type). Enzymatic deglycosylation reduces this broad band to a single, sharp 29 kDa band.
C. Western Blot Analysis
ADG3937 is suitable for use in Western blot analysis. Under non-reducing conditions, 2 μg/mL of ADG3937 can visualize as little as 50 ng of uPAR.

Category:
Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

For more information please click .pdf icon below.


murine MAb against human uPAR, clone HD-UPAR-13.1

Cat.No. ADG3937
Article no.: 938132

Unit: 250 µg

Code: ADG3937

Manufacturer: ImmBioMed GmbH & Co. KG

References

  1. Urokinase system expression in gastric carcinoma: prognostic impact in an independent patient series and first evidence of predictive value in preoperative biopsy and intestinal metaplasia specimens. Beyer et al., Cancer. 2006 Mar 1;106(5):1026-1035.
  2. uPA-Silica-Particles (SP-uPA): A Novel Analytical System to Investigate uPA-uPAR Interaction and to Test Synthetic uPAR Antagonists as Potential Cancer Therapeutics. Guthaus et al., 2002, Biol. Chem., Vol. 383, pp. 207–216.
  3. Epitopes of components of the plasminogen activation system are re-exposed in formalin-fixed paraffin sections by different retrieval techniques. Ferrier CM et al., J Histochem Cytochem. 1998 Apr;46(4):469-476.
  4. Semi-quantitation of urokinase plasminogen activator and its receptor in breast carcinomas by immunocyto-chemistry. Kennedy et al., Br J Cancer. 1998 May; 77(10): 1638–1641. 
  5. Dispase-Mediated Basal Detachment of Cultured Keratinocytes Induces Urokinase-type Plasminogen Activator (uPA) and Its Receptor (uPA-R, CD87). Schaefer et al., 1996, Exp Cell Res 228, 246–253.
  6. Upregulation of Cell-Surface-Associated Plasminogen Activation in Cultured Keratinocytes by Interleukin-1b and Tumor Necrosis Factor-a. Bechtel et al., 1996. Exp Cell Res 223, 395–404.

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