anti-human CD62P, PE-conjugated, Clone HI63P
Description
CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.
Properties
The monoclonal antibody ADG5066/L (clone HI62P) is a murine monoclonal antibody, subclass IgG1. The antibody has been purified from ascites using protein-G affinity chromatography, Purity > 95% (by SDS-PAGE).
The antibody recognizes a 140 kDa long single chain type I transmembrane glycoprotein, P-¬selectin, also called Granule membrane protein-140 (GMP-140) or platelet activation dependent granule¬ external membrane protein (PADGEM). CD62P antigen is present in the α-granules of platelets and in Weibel-Palade bodies of endothelial cells, and is rapidly mobilized from storage granules to the cell surface after cell activation. So, CD62P is expressed on the surface of the activated platelets. Interaction of CD62P with PSGL-1 (CD162) mediates tethering and rolling of leukocytes on the surface of activated endothelial cells, the first step in leukocyte extravasation and migration towards sites of inflammation. It also mediates rolling of platelets on endothelial cells and CD62P-mediated interactions are also involved in platelet-mediated delivery of lymphocytes to high endothelial venules (HEV).
Conjugation
The purified antibody is conjugated with R-Phycoerythrin (R-PE) under optimum conditions. The reagent is adjusted for direct use. No reconstitution is necessary.
Presentation
Vial containing 500 µl (ADG5066) or 2 ml (ADG5066L) of purified antibody in PBS containing 1% BSA and 0.09% sodium azide (pH 7.2). The IgG concentration is 1 mg/ml. Spin the vial briefly before opening.
Storage and Stability
Store the antibody at 4°C. Avoid prolonged exposure to light. The reagent is stable until the expiry date stated on the vial label.
Applications
Flow Cytometry
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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anti-human CD62P, PE-conjugated, Clone HI62P
Cat.No. ADG5066
Article no.: 938167
Unit: 500 µl
Code: ADG5066
Manufacturer: ImmBioMed GmbH & Co. KG
References
- Ramachandran V, Williams M, Yago T, Schmidtke DW, McEver RP: Dynamic alterations of membrane tethers stabilize leukocyte rolling on P-selectin. Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13519-24.
- Martinez M, Joffraud M, Giraud S, Baïsse B, Bernimoulin MP, Schapira M, Spertini O: Regulation of PSGL-1 interactions with L-selectin, P-selectin, and E-selectin: role of human fucosyltransferase-IV and -VII. J Biol Chem. 2005 Feb 18;280(7):5378-90.
- Harakawa N, Shigeta A, Wato M, Merrill-Skoloff G, Furie BC, Furie B, Okazaki T, Domae N, Miyasaka M,
Hirata T. P-selectin glycoprotein ligand-1 mediates L-selectin-independent leukocyte rolling in high endothelial venules of peripheral lymph nodes. Int Immunol. 2007 Mar;19(3):321-9.