murine MAb against human plasminogen and antiplasmin complexes
Description
Full length Plasminogen comprises seven domains, a C-terminal chymotrypsin-like serine protease domain, a N-terminal Pan Apple domain (PAp) and five Kringle domains (KR 1-5).
Plasminogen is synthesised in the liver and circulates in two forms: Glu-Plasminogen and Lys-Plasminogen. In its native form Plg contains a glutamic acid residue at the N-terminus and this molecule is termed Glu-Plasminogen.
Native Glu-Plasminogen (88 KDa) is readily converted to Lys-plasminogen (83 KDa) by Plasmin hydrolysis of the Lys76-Lys77 peptide bond. PMN-Elastase catalyzed cleavage of the Val441-Val442 peptide bond of plasminogen yields a functionally active zymogen termed Val-442-Plasminogen or mini-Plasminogen, which contains Kringle 5 and the proteolytic domain.
Properties
The monoclonal antibodies were generated against purified human Plasminogen. The antibodies have been purified from murine ascites by Protein A affinity chromatography.
This antibody (clone PG7, isotype IgG1) reacts with free plasminogen and plasmin in alpha-2-antiplasmin complexes, as determined per Elisa. The antibody is directed against an epitope on the kringle 4 elastase fragment of plasminogen. (1)
Summary
Binding to Plasminogen: KR 4
binding to Plasmin-alpha2-antiplasmin complex: positive
Half-maximal binding to coated Plasminogen: negative
Inhibition of Plaminogen activation: negative
Stimulation of Plasminogen activation: positive
Presentation
Vials containing 0.5 mg IgG, lyophilized from 0.01 M potassium phosphate, 0.14 M sodium chloride with 0.02% sodium azide and 20 mg/ml mannitol added.
Reconstitution
Add 0.5 mL filtered deionized water to generate a 1.0 mg/mL stock solution.
Storage and Stability
Store lyophilized antibodies at 2°-8°C. Aliquot and store reconstituted antibodies at -20°C or colder. It is recommended to avoid freeze-thaw cycles.
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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References
- Monoclonal antibodies against plasminogen and
α-2-antiplasmin: Binding to native and modified antigens. Hattey E et al., Thromb Res 1987, 45: 485.
- Monoclonal antibodies against four different epitopes of plasminogen exhibit different effects on plasminogen activation kinetics. Hattey E et al., Thromb Haemost 1987, 58, No.1: 440, Abstr. 1622.
- Maywood II: A Congenitally Abnormal Plasminogen with Defective Lysine-Binding Sites Associated with Stroke in a 16-Year-Old Male. Kozlowski KA, et al., Fibrinolysis 1995, 9: 17-27.