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murine MAb against the 41-kDa flagellin of Borrelia burgdorferi, Clone LA-85.1

Description
Lyme disease is the most common vector-borne disease in North America and Europe. The causative agent Borrelia burgdorferi is a bacterium that is maintained in an enzoonotic cycle between Ixodes ticks and a large range of mammals. The 41 kDa flagellin of Borrelia burgdorferi is a major component of periplasmic flagellar filament core and a good candidate for serodiagnosis in early stage of Lyme disease.

Properties
The monoclonal antibody ADG0094L (clone LA-85.1) is a murine monoclonal antibody, subclass IgG2a recognizing flagellin. Mice were immunized with cell lysates of Borrelia burgdorferi. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.

Presentation
Vial containing 1 mg purified antibody in PBS pH 7.4. The concentration is given on the vial label. Spin the vial briefly before opening.

Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.

Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Westernblot
The antibody is suitable for Western blot analysis, detecting native and recombinant lpLA7 following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Immunocytochemistry
The antibody can be used for immunocytochemistry on paraformaldehyde fixed.

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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murine MAb against the 41-kDa flagellin of Borrelia burgdorferi, Clone LA-85.1

Cat.No. ADG0094L (research box)
Article no.: 938288

Unit: 1,0 mg

Code: ADG0094L

Manufacturer: ImmBioMed GmbH & Co. KG - from the research box

References

  1. Characterization of Borrelia burgdorferi associated antigens by monoclonal antibodies. Kramer et al. Immunobiol. 1990; 181:357-366
  2. The Borrelia burgdorferi flagellum-associated 41-kilodalton antigen (flagellin): molecular cloning, expression, and amplification of the gene. Wallich et al. Infect. Immun. 1990; 58(6) 1711-1719
  3. Cellular immune reactivity to recombinant OspA and flagellin from Borrelia burgdorferi in patients with Lyme borreliosis. Complexity of humoral and cellular immune responses. Krause et al. J. Clin. Invest. 1992; 90(3):1077-1084
  4. Antigenic variation and strain heterogeneity in Borrelia spp. Wilske et al. Res. Microbiol. 1992; 143(6):583-596
  5. Evaluation of genetic divergence among Borrelia burgdorferi isolates by use of OspA, fla, HSP60, and HSP70 gene probes. Wallich et al. Infect. Immun. 1992; 60(11):4856-4866

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