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murine MAb against HSP70 of Borrelia burgdorferi, Clone LA-3

Description
Lyme disease is the most common vector-borne disease in North America and Europe. The causative agent Borrelia burgdorferi is a bacterium that is maintained in an enzoonotic cycle between Ixodes ticks and a large range of mammals. The 66- to 73-kDa proteins of Borrelia burgdorferi are dominant immunogens and expressed in all strains of B. burgdorferi. The amino terminal sequence of the 70-kDa HSP70 proteins of B. burgdorferi are almost identical and exhibit remarkable sequence similarity to the DnaK heat-shock protein of E. coli.

Properties
The monoclonal antibody ADG0109L (clone LA-3) is a murine monoclonal antibody, subclass IgG2a recognizing HSP70. Mice were immunized with cell lysates of Borrelia burgdorferi. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.

Presentation
Screw capped vial containing 1 mg of purified antibody in PBS pH 7.4. The IgG concentration is given on the vial label. Spin the vial briefly before opening.

Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.

Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Westernblot
The antibody is suitable for Western blot analysis, detecting native and recombinant HSP70 following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Immunocytochemistry
The antibody can be used for immune-cytochemistry on paraformaldehyde fixed spirochetes and for cryo-electron tomography.

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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murine MAb against HSP70 of Borrelia burgdorferi, Clone LA-3

Cat.No. ADG0109L (research box)
Article no.: 938290

Unit: 1,0 mg

Code: ADG0109L

Manufacturer: ImmBioMed GmbH & Co. KG - from the research box

References

  1. Characterization of Borrelia burgdorferi associated antigens by monoclonal antibodies. Kramer et al. Immunobiol. 1990; 181:357-366
  2. Evaluation of genetic divergence among Borrelia burgdorferi isolates by use of OspA, fla, HSP60, and HSP70 gene probes. Wallich et al. Infect. Immun. 1992; 60(11):4856-4866
  3. Novel Borrelia burgdorferi isolates from Ixodes scapularis and Ixodes dentatus ticks feeding on humans. Anderson et al. J. Clin. Microbiol. 1996; 34(3):524-529
  4. Borrelia burgdorferi HSP70 homolog: characterization of an immunoreactive stress protein. Anzola et al. Infect. Immun. 1992; 60(9):3704-3713
  5. Immunological and structural characterization of the dominant 66- to 73-kDa antigens of Borrelia burgdorferi. Luft et al. J. Immunnol. 1991; 146:2776-2782
  6. Characterization of the heat shock response and identification of heat shock protein antigens of Borrelia burgdorferi. Carreiro et al. Infect. Immun. 1990; 58:2186-2191
  7. Coordinate synthesis and turnover of heat shock proteins in Borrelia burgdorferi: degradation of DnaK during recovery from heat shock. Cluss et al. Infect. Immun. 1996; 64(5):1736-1743

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