goat anti-human PAI-1 IgG
Description
Plasminogen Activator Inhibitor Type 1 (PAI-1) is a member of the serpin family of protease inhibitors. It functions as the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA) nd hence is an inhibitor of fibrinolysis.
PAI-1 is a single-chain glycoprotein with a molecular weight of 47 kDa. It is mainly produced by the endothelium (cells lining blood vessels), but is also secreted by other tissue types, such as adipose tissue.
Preparation
Goats were immunized with recombinant human PAI-1. The IgG fraction of immunserum was isolated by affinity chromatography on Protein G-Sepharose.
Presentation
Screw-capped clear glass vial containing 1.0 mg of purified IgG lyophilized from 1 mL PBS with 200 mM mannitol, pH 7.5.
Reconstitution
Add 1 mL of filtered deionized water. Agitate gently for 5 minutes.
Storage and Stability
Store unopened vials at 2 - 8 °C until the expiration date shown on label.
Store reconstituted antibody in aliquots at -20 °C or colder. Stable for at least 6 months; avoid repeated freeze and thaw cycles.
Applications
ELISA
The antibody can be used as capture or detection antibody in ELISAs.
Western Blot
The antibody can be used for immuno-blotting. It recognizes native (a) and recombinant (b) PAI-1.
Immunohistochemistry
The antibody can be used for immunohistological staining of frozen and formalin-fixed, paraffin-embedded tissue sections.
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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goat anti-human PAI-1 IgG
Cat.No. ADG75
Article no.: 938530
Unit: 1 mg
Code: ADG75
Manufacturer: ImmBioMed GmbH & Co. KG
References
- Plasminogen activator inhibitor-1 and prognosis in primary breast cancer. Foekens JA et al., J Clin Oncol. 1994 Aug;12(8):1648-1658.
- Immunohistochemical characterization of the plasminogen activator system in psoriatic epidermis. Gissler HM et al., Br J Dermatol. 1993 Jun;128(6):612-618.
- alpha 2-Antiplasmin and plasminogen activator inhibitors in healing human skin wounds. Schaefer BM et al., Arch Dermatol Res. 1996 Mar;288(3):122-128.