OLIGOBIND® Thrombin Inhibitor solution
DESCRIPTION
OLIGOBIND® Thrombin inhibitor solution is intended for the collection of whole blood for further preparation and assaying active Thrombin (TH) in plasma. The solution contains reversible active site inhibitors of TH. The inhibitor protects TH from inhibition by plasma protease inhibitors in drawn blood ex vivo.
The solution is intended for research use only.
USAGE
Add the OLIGOBIND® TH inhibitor solution to the sodium citrate blood collection tube. The inhibitor has to be diluted 1+200 in the tubes (e.g for blood collection tube with a fill size of 3 ml add 150 µl inhibitor solution).
EQUIPMENT REQUIRED BUT NOT PROVIDED
Sodium citrate blood collection tubes (0,106 mol/l).
PRESENTATION
OLIGOBIND® TH inhibitor solution 2.2 ml (2x 1.1 ml vials). Suitable for 25x 3ml blood collection tubes.
Recommendation for blood collection
1. Collect blood into Blood Collection Tubes containing sodium citrate and the inhibitor solution:
- puncture vein by using sterile blood collection system, such as a wing canula with tubing (“butterfly”)
and appropriate Luer-Lock adapters
- note: the first blood draw (up to 3 ml) should not be used for APC determination, therefore use
other blood collection tube (up to 3 ml), e.g. for other analysis than APC determination.
- then immediately draw blood into APC Blood Collection Tube(s).
- discard material in case of initially unsuccessful puncture attempt or obviously impaired blood flow
during sampling.
- do not puncture the same vein again but preferably chance to other limb for second attempt.
2. Drawn blood should be stored cooled (4 °C) and centrifuged (see step 3) within 4 hours.
3. Centrifuge the blood sample at 2,500 x g for 15 minutes.
4. Plasma should be stored at 2°- 8°C and assayed within 4 hours.
Alternatively, plasma may be stored at -20°C for up to 6 months.
5. Frozen plasma should be thawed rapidly at 37°C.
Follow the general guidelines for plasma preparation and handling of potentially infectious specimens.
Blood-to-additive variation can cause erroneous results if the tubes are over filled or under filled.
STORAGE AND STABILITY
Store OLIGOBIND® APC Inhibitor solution at 2°-8°C. Solution may be used until the recorded expiration date. Shipment of product does not require cooling during the time of transportation.
Category: Research use only
Type: Inhibitor Solution for ADG844 OLIGOBIND Thrombin activity assay
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
For more information please click .pdf icon below.
OLIGOBIND Thrombin Inhibitor solution
Cat.No. ADG844IS
Article no.: 938539
Unit: 2,2 ml
Code: ADG844IS
Manufacturer: ImmBioMed GmbH & Co. KG
BIBLIOGRAPHY
- Thrombin inhibition profiles in healthy individuals and thrombophilic patients. Rühl H, Müller J, Harbrecht U, Fimmers R, Oldenburg J, Mayer G, Pötzsch B. Thromb Haemost. 2012; 107: 848-853.
- Profiling of active thrombin in human blood by supramolecular complexes. Müller J, Becher T, Braunstein J, Berdel P, Gravius S, Rohrbach F, Oldenburg J, Mayer G, Pötzsch B. Angew Chem (Int Ed Engl) 2011 Jun 27;50(27):6075-6078.
- Measurement of free thrombin in human plasma using an oligonucleotide-based enzyme capture assay (OECA). Becher T, Müller J, Braunstein J, Mayer G, Pötzsch B. Haemostasiologie, 2010, 30 1: P17-02.
- A new measure for in vivo thrombin activity in comparison with in vitro thrombin generation potential in patients with hyper- and hypoc-oagulability. Königsbrügge O, Silvia Koder S, Riedl J, Panzer S, Pabinger I, and Ay C. Clin Exp Med. 2017 May;17(2):251-256.
- Activity pattern analysis indicates increased but balanced systemic coagulation activity in response to surgical trauma. Friedrich MJ, Schmolders, Rommelspacher Y, Strauss A, Rühl H, Mayer G, Oldenburg J, Wirtz DC, Müller J, Pötzsch. TH Open 2018; 2: e350-e356.