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Pefachrome® tPA, Chromogenic Substrate for tPA

Application:
Highly sensitive chromogenic peptide substrate for tissue-type plasminogen activator (tPA). Determination of tPA activity for research, in-process and quality control. Pefachrome. tPA shows different sensitivity to native single-chain tPA (sc-tPA) and its fully active two-chain form (tc-tPA). This allows a differential determination of each molecular form. 

Formula: CH3SO2-D-CHA-Gly-Arg-pNA · AcOH 

Principle:
CH3SO2-D-CHA-Gly-Arg-pNA + E => CH3SO2-D-CHA-Gly-Arg-OH + pNA + E
E = Enzyme (tc-tPA or sc-tPA) 

MW: 658.9 

Kinetic data:
  KM Vmax
sc-tPA 0.286 mM 6.95 nmol/µg tPA/min
tc-tPA      0.167 mM    33.9 nmol/µg tPA/min  
 
Storage:
May be used by the expiry date given on the label when stored unopened, protected from moisture, in the dark, 2-8°C. Avoid contamination of the reagents by micro-organisms. Shipment of product does not require cooling during the time of transportation.

Material required but not provided:
Buffer, reference material

Buffer: 50 mM Tris-Imidazole buffer pH 8.4, 300 mM NaCl, 37 °C

Assay:
Suggested protocol for the determination of native single-chain tPA (sc-tPA):
1.780 ml buffer
0.020 ml Enzyme (approx. 75’000 IU /ml)
0.200 ml Pefachrome®tPA (2 mM in water)
=> Determination of ∆OD/min at 405 nm

Suggested protocol for the determination of two-chain tPA (tc-tPA):
1.750 ml buffer
0.050 ml Enzyme (approx. 120’000 IU /ml)
0.200 ml Pefachrome®tPA (4 mM in water)
=> Determination of ∆OD/min at 405 nm

Category: Research use only

Type: Substrate

Product Availability: Germany, Israel and Egypt

Manufacturer: DSM Nutritional Products Ltd. Branch Pentapharm, Switzerland


For more information please click .pdf icon below.


Pefachrome tPA

Chromogenic Substrate for tPA
Article no.: 809101/25

Unit: 25 mg

Code: 091-01

Manufacturer: Pentapharm AG

Reference

  1. Kluft C, ed.
    Tissue type Plasminogen Activator (tPA): Physiological and Clinical Aspects. Boca Raton: CRC Press; 1988: Vol.I 123-144, Vol.II 211-224 

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